Synergy of tendon stem cells and platelet-rich plasma in tendon healing.

نویسندگان

  • Lei Chen
  • Shi-Wu Dong
  • Jun-Peng Liu
  • Xu Tao
  • Kang-Lai Tang
  • Jian-Zhong Xu
چکیده

Injured rat Achilles tendons were treated with botulism toxin to create a mechanically unloaded condition (unloaded) or left untreated (loaded), and then treated with phosphate-buffered saline (PBS), platelet-rich plasma (PRP), tendon stem cells (TSCs), or a combination (TSCs + PRP). mRNA and protein expression of collagen I, collagen III, tenascin C, and Smad 8 were determined by real time PCR and immunostaining, respectively. Loaded tendons treated with PBS, PRP, or TSCs for 3 or 14 days had higher collagen I mRNA expression than unloaded tendons. Loaded tendons treated with PBS for 3 or 14 days or with PRP for 3 days had higher collagen I protein levels than unloaded tendons. Loaded tendons treated for 3 days with PBS, for 14 days with PRP or TSCs or TSCs + PRP for 3 or 14 days had higher collagen III protein levels than unloaded tendons. Collagen I mRNA levels were higher in TSCs + PRP-treated loaded tendons compared to PBS-treated loaded tendons on day 3 of treatment. Based on changes in the expression of tendon-healing genes, our data suggest that the combination of TSCs and PRP has synergistic effects on tendon healing under both loaded and unloaded conditions, and loaded conditions improve tendon healing.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The effect of aquatic activity and alogenic bone marrow derived mesenchymal stem cells fortified with Platelet-Rich Plasma in treatment of Achilles tendon in rat

The aim of this study was to the effect of aquatic activity and alogenic bone marrow derived mesenchymal stem cells fortified with Platelet-Rich Plasma in treatment of Achilles tendon in rat.  74 Sprague-Dawley rats were selected and tendon injury was formed in 69 of them. Subsequently, these rats were randomly divided into 8 groups and 5 rats which were without any injuries were chosen as the ...

متن کامل

Temporal and Spatial Expression of TGF-b1 in the Early Phase of Patellar Tendon Healing after Application of Platelet Rich Plasma

   Background:The aim of this study is to find out the spatial and temporal expression of TGF-b1 during the tendon healing, after application of Platelet Rich Plasma (PRP). Methods: A patellar tendon defect model in rabbits was used for this purpose. 48 skeletally mature New Zealand White rabbits, weighing 3.5 kg, were used for this study. Equal numbers of animals from both groups were sacrific...

متن کامل

Comparison of Platelet-Rich Plasma (PRP), Bone Marrow-Derived Mesenchymal Stem Cells‏ and their Combination on the Healing of Achilles tendon in Rabbits

Objectives- The purpose of the study was to observe whether PRP, Mesenchymal Stem Cells (MSCs) and combining them could help make improvements the healing of Achilles tendons in rabbits. Design- Experimental study. Animals-Sixteen male, healthy and mature white New Zealand rabbits. Procedures- The animals were...

متن کامل

The Healing Effects of Aquatic Activities and Allogenic Injection of Platelet-Rich Plasma (PRP) on Injuries of Achilles Tendon in Experimental Rat

BACKGROUND Clinical tendon injuries represent serious and unresolved issues of the case on how the injured tendons could be improved based on natural structure and mechanical strength. The aim of this studies the effect of aquatic activities and alogenic platelet rich plasma (PRP) injection in healing Achilles tendons of rats. METHODS Forty rats were randomly divided into 5 equal groups. Se...

متن کامل

Evaluation of the Effect of Platelet Rich Plasma (PRP) in Tendon Gap Healing by Measuring Collagen Synthesis in Guinea Pig

Objectives- Hydroxyproline is a specific amino acid of collagen which is widely used to estimate the collagen content in biological specimens. The purpose of this study was to compare the effect of the Platelet Rich Plasma (PRP) on tendon gap healing using two different scaffold made from subcutaneous fascia and polypropylene tube. Design- Experimental study Animals - 16 young adult Guinea pigs...

متن کامل

Use of Platelet Rich Plasma and Hyaluronic Acid in the Treatment of Complications of Achilles Tendon Reconstruction

BACKGROUND The platelet-rich plasma (PRP) and hyaluronic acid (HA) constitute a system of tissue growth that can regenerate damaged tissue. This study was performed to evaluate the effect of PRP and HA in treatment of complications of Achilles tendon reconstruction. METHODS We selected ten patients affected by Achilles tendon injuries resulting from post-surgical complications subseque...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Journal of orthopaedic research : official publication of the Orthopaedic Research Society

دوره 30 6  شماره 

صفحات  -

تاریخ انتشار 2012